Work

Targazyme – Carlsbad, CA - Nov. 2016 - present

Senior Translational Scientist – Finding new clinical applications for enzyme technology

  • Finding new indications for pipeline of enzymes (TZ101, TZ102) in hematopoietic stem cell transplantation, immunotherapy (NK, CAR-T), and regenerative medicine for translation into clinical applications
  • Fundraising for clinical trials and new projects through grants (NIH SBIR, R01, CIRM), angel investing, and crowdfunding
  • Engaged in business and clinical development

Targazyme – Carlsbad, CA - Jul. 2016 - Oct. 2016

Consultant – Fundraising for clinical trials for TZ101 in stem cell transplantation

Nano3D Biosciences – Houston, TX - Mar. 2013 - Sep. 2016

Sr. Scientist, R&D – Developing new applications for magnetic 3D bioprinting

  • Designing rapid and predictive assays and tools for high-throughput drug toxicity/efficacy screening
  • Expanding applications into fields of interest, such as cardiotoxicity and hepatotoxicity 
  • Aiding efforts in marketing and sales: website and graphic design, exhibiting at trade shows, presentations, product demonstrations, technical support
  • Gaining experience in business: meeting with investors, writing business plans, identifying key markets, competitors, and opportunities
  • Presented results on bioprinting, regenerative medicine, and high-throughput at conferences in US, Singapore, France
  • Helped win 2 NIH SBIR Phase I grants, PI on one
  • Published 7 peer-reviewed papers

University of Texas Health Science Center at Houston – Houston, TX - Oct. 2015 - Sep. 2016

Adjunct Assistant Professor, Department of Internal Medicine, Division of Oncology

  • Collaborating on precision oncology assay development using patient-derived cells from solid tumor and liquid biopsies for NGS to personalize cancer treatment
  • Developing precision assay to personalize tocolytic therapy for preterm labor management
  • Developed novel method to deliver stem-cell therapy for erectile dysfunction

Nano3D Biosciences – Houston, TX - Jun. 2010 - Dec. 2010

Research Intern – Found new applications and techniques for 3D levitated cell culture using magnetic nanoparticles

  • Designed new tools and methods for immunohistochemistry and co-culture of 3D magnetically levitated cells
  • Developed a co-culture model of the bronchiole and the aortic valve for drug toxicity testing and research
  • Presented results at conferences in biomedical engineering and submitting publication in prominent journal on tissue engineering
  • Helped prepare NSF SBIR Phase II Report
  • Managed a research team to execute research goals and publish results
  • Gained valuable experience in start-up culture

 

Education

Rice University, Houston, TX - Aug. 2007 - Mar. 2013

PhD in Bioengineering · Advised by K. Jane Grande-Allen, PhD, Isabel C. Cameron Professor of Bioengineering · GPA 3.46

Johns Hopkins University (JHU), Baltimore, MD - Sept. 2003 - May 2007

BS with double major in Engineering Mechanics, Applied Mathematics & Statistics · GPA 3.60

 

Research

Integrative Matrix Mechanics Laboratory, Department of Bioengineering, Rice University , Houston, TX Jan. 2008 - Mar. 2013

Graduate Student - Research spans tissue engineering, biomechanics, biomaterials, and cardiovascular fields 

  • Characterized the microstructure of the aortic valve leaflet for tissue engineering applications
  • Designed a novel laminate composite hydrogel scaffold for heart valve tissue engineering
  • Developed an innovative 3D co-culture model of the aortic valve for life science research applications
  • Published 9 peer-reviewed papers and 1 book chapter
  • Presented results at conferences in biomedical engineering, biomaterials, and heart valves in US, China, Greece
  • Managed a lab of up to 13 members as senior member and mentored eight undergraduate research assistants on separate research projects
  • Helped win $1.2 million NIH R01 grant for heart valve tissue engineering

JHU Applied Physics Laboratory – Columbia, MD - Jan. 2007 - Jun. 2007

Advised by Mehran Armand, PhD

Research Assistant - Orthopedics research and computer-integrated surgery

  • Worked with orthopedic surgeons and professional engineers
  • Developed a surgical training program in MATLAB for surgical residents to practice total hip arthroplasties
  • Designed user interface for users to plan and view surgical path in 2D and 3D

Hong Kong University, Dept. of Orthopaedics and Traumatology - Hong Kong SAR, China - Jun. 2006 - Aug. 2006

Advised by William W. Lu, PhD, Professor of Orthopaedics and Traumatology

Research Assistant - Orthopedics and biomaterials research

  • Assessed the viability of novel bioactive strontium-hydroxyapatite (Sr-HA) bone cement at bone-screw interfaces
  • Found that insertion timing did not change implant strength with Sr-HA compared to traditional PMMA cement
  • Gained experience collaborating in a different culture and language

JHU Bayview Medical Center - Baltimore, MD - Oct. 2005 - Dec. 2006

Advised by Stephen Belkoff, PhD, Associate Professor of Orthopedic Surgery, Mechanical Engineering

Research Assistant - Collaborated with industry sponsors to test mechanics and kinematics of spinal implants

  • Designed apparatuses to adapt conventional uniaxial mechanical testers to mimic physiological motion
  • Presented data as feedback to sponsors on mechanical design

 

Publications

Desai PK, Tseng H, Souza GR. Assembly of hepatocyte spheroids using magnetic 3D cell culture for CYP450 inhibition/induction. Int J Mol Sci, 2017; 18(5):1085. PDF

Souza GR, Tseng H, Gage JA, Mani A, Desai P, Leonard F, Liao A, Longo M, Refuerzo JS, Godin B. Magnetically bioprinted human myometrial 3D cell rings as a model for uterine contractility. Int J Mol Sci, 2017; 18(4):683. PDF

Tseng H, Gage JA, Haisler WL, Neeley SK, Shen T, Hebel C, Barthlow HG, Wagoner M, Souza GR. A high-throughput in vitro ring assay for vasoactivity using magnetic 3D bioprinting. Sci Rep, 2016; 6:30460. PDF

Lin H, Dhanani N, Tseng H, Souza GR, Wang G, Cao Y, Ko TC, Jiang H, Wang R. Nanotechnology improved stem cell therapy for erectile dysfunction in a rat model of cavernous nerve injury. J Urol, 2016; 3:788-95. PDF

Tseng H, Gage JA, Shen T, Haisler WL, Neeley SK, Shiao S, Chen J, Desai PK, Liao A, Hebel C, Raphael RM, Becker JL, Souza GR. A spheroid toxicity assay using magnetic 3D bioprinting and real-time mobile device-based imaging. Sci Rep, 2015; 5:13987. PDF

Balaoing LR, Post AD, Lin AY, Tseng H, Moake JL, Grande-Allen KJ. Laminin peptide-immobilized hydrogels modulate valve endothelial cell hemostatic regulation. PLoS One, 2015; 10(6):e0130749. PDF

Zhang X, Xu B, Yonezawa AL, Tseng H, Puperi DS, Wu Y, Cuchiara ML, West JL, Grande-Allen KJ. Integrating valve-inspired design principles into poly(ethylene glycol) hydrogel scaffolds for heart valve tissue engineering. Acta Biomater, 2015; 14:11-21. PDF

Tseng H, Puperi DS, Kim EJ, Ayoub S, Shah JV, Cuchiara ML, West JL, Grande-Allen KJ. Anisotropic poly(ethylene glycol)/polycaprolactone (PEG/PCL) hydrogel-fiber composites for heart valve tissue engineering. Tissue Eng Part A, 2014; 20(19-20):2634-2645. PDF

Tseng H, Balaoing LR, Grigoryan B, Raphael RM, Killian TC, Souza GR, Grande-Allen KJ. A three-dimensional co-culture model of the aortic valve using magnetic levitation. Acta Biomater, 2014; 10(1):173-82. PDF

Timm DM, Chen J, Sing D, Gage JA, Haisler WL, Neeley SK, Raphael RM, Dehghani M, Rosenblatt KP, Killian TC, Tseng H, Souza GR. A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis. Sci Rep, 2013; 3:3000.  PDF

Haisler WL, Timm DM, Gage JA, Tseng H, Killian TC, Souza GR. Three-dimensional cell culturing by magnetic levitation. Nat Protoc, 2013; 8(10):1940-9. PDF

Tseng H, Gage JA, Raphael RM, Moore RH, Killian TC, Grande-Allen KJ, Souza GR. Assembly of a three-dimensional multitype bronchiole co-culture model using magnetic levitation. Tissue Eng Part C Methods, 2013; 19(9):655-75.  PDF

Tseng H, Kim EJ, Connell PS, Ayoub S, Shah JV, Grande-Allen KJ. The tensile and viscoelastic properties of aortic valve leaflets treated with a hyaluronidase gradient. Cardiovasc Eng Technol, 2013; 4(2):151-60. PDF

Tseng H, Cuchiara ML, Durst CA, Cuchiara MP, Lin CJ, West JL, Grande-Allen KJ. Fabrication and mechanical evaluation of anatomically-inspired quasilaminate hydrogel structures with layer-specific formulations. Ann Biomed Eng, 2012; 41(2):398-407. PDF

Tseng H, Grande-Allen KJ. Elastic fibers in the aortic valve spongiosa: A fresh perspective on its structure and role in overall tissue function. Acta Biomater. 2011; 7(5):2101-8. PDF

Ferdous Z, Peterson SB, Tseng H, Anderson DK, Iozzo RV, Grande-Allen KJ. A role for decorin in controlling proliferation, adhesion, and migration of murine embryonic fibroblasts. J Biomed Mater Res A. 2010; 93(2):419-28. PDF

Tseng H, Grande-Allen KJ. Microstructure of the aortic valve: implications for valvular mechanics and function. Advances in Cardiovascular Research, Volume 1. Schmitt L, Konig T, editors. Hauppauge, NY: Nova Science Publishers; 2010.

Gupta V, Tseng H, Lawrence BD, Grande-Allen KJ. Effect of cyclic mechanical strain on glycosaminoglycan and proteoglycan synthesis by heart valve cells. Acta Biomater. 2009; 5(2):531-540. PDF

 

Posters and Presentations

Tseng H, Dehghani M, Ionescu-Zanetti C, Gage JA, Desai PK, Liao A, Brobey R, Jensen J, Rosenblatt KP, Souza GR, Amato RJ. Somatic mutation detection from liquid biopsy-derived cellular aggregates formed by magnetic 3D bioprinting. AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics, Boston, MA, 2015.

Amato R, Brobey R, Dehghani M, Rosenblatt K, Souza G, Tseng H, Jensen J, Ionescu-Zanetti C. Somatic mutation detection via sequencing using circulating tumor cell samples from patients with renal cell and prostate cancer. AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics, Boston, MA, 2015.

Tseng H. High-throughput functional toxicity screening using iPS-cardiomyocyte and hepatocyte spheroids by magnetic 3D bioprinting (podium). iForum, Chicago, IL, 2015.

Tseng H, Desai PK, Gage JA, Haisler WL, Liao A, Honsich U, Velkova-Krei A, Souza GR, Seldin J. High-throughput functional toxicity screening using iPS-cardiomyocyte and hepatocyte spheroids by magnetic 3D bioprinting (poster). iForum, Chicago, IL, 2015.

Tseng H. Magnetic 3D bioprinting. Seminar, Baylor College of Medicine, Houston, TX, 2015. 

Tseng H, Gage JA, Haisler WL, Desai PK, Souza GR. High-throughput spheroid formation in a 384-well format using magnetic 3D bioprinting. American Association of Cancer Research Annual Meeting, Philadelphia, PA, 2015. 

Tseng H, Gage JA, Haisler WL, Desai PK, Souza GR. High-throughput spheroid printing and toxicity testing using magnetic 3D bioprinting. Society of Lab Automation and Screening Conference and Exhibition, Washington, DC, 2015. 

Tseng H. High-throughput compound screening with magnetic 3D bioprinting. High-Throughput Cell Biology: From Screening to Applications, Paris, France, 2014.

Tseng H, Gage JA, Haisler WL, Neeley SK, Souza GR, Killian TC. Magnetic 3D Bioprinting: A novel high-throughput and high-content assay for toxicity screening. 2014 Regenerative Medicine Symposium, Houston, TX, 2014. 

Tseng H. Magnetic 3D bioprinting for high-throughput compound screening. International Bioprinting Congress, Singapore, 2014.

Tseng H. Magnetic 3D bioprinting for high-throughput compound screening. Seminar, Universiti Tunku Abdul Rahman (UTAR), Petaling Jaya, Malaysia, 2014.

Tseng H. Magnetic 3D Bioprinting: Applying biomaterials for high-throughput compound screening. Biomaterials Day, College Station, TX, 2014.

Tseng H, Gage JA, Haisler WL, Neeley SK, Souza GR, Killian TC. Magnetic 3D Bioprinting: A novel high-throughput and high-content assay for toxicity screening. European Society of Toxicology In Vitro International Conference, Egmond aan Zee, The Netherlands, 2014.

Tseng H, Gage JA, Haisler WL, Neeley SK, Souza GR, Killian TC. Magnetic 3D Bioprinting: A novel high-throughput and high-content assay for toxicity screening. Society of Toxicology Annual Meeting. Phoenix, AZ, 2014.

Tseng H, Timm DM, Chen J, Haisler WL, Gage JA, Neeley SK, Raphael RM, Killian TC, Souza GR. A high-throughput three-dimensional cell migration assay (BiO Assay) for toxicity screening for breast cancer applications. San Antonio Breast Cancer Symposium. San Antonio, TX, 2013.

Tseng H, Timm DM, Chen J, Haisler WL, Gage JA, Neeley SK, Raphael RM, Killian TC, Souza GR. A high-throughput three-dimensional cell migration assay for toxicity screening using magnetic levitation with mobile device-based macroscopic image capture. American Association of Pharmaceutical Scientists Annual Meeting and Exposition. San Antonio, TX, 2013.

Tseng H, Souza GR. The BiO Assay: A 3D high-throughput cell migration and viability assay using an iPod Touch. Advanced Models for Imaging-Based Drug Screening. Houston, TX, 2013.

Tseng H, Gage JA, Moore RH, Raphael RM, Killian TC, Grande-Allen KJ, Souza GR. Assembly of a three-dimensional multitype bronchiole co-culture model using magnetic levitation. Smalley Curl Celebration. Houston, TX, 2013.

Balaoing LR, Tseng H, Grigoryan B, Raphael RM, Killian TC, Souza GR, Grande-Allen KJ. A three-dimensional co-culture model of the aortic valve using magnetic levitation. Society for Biomaterials Annual Meeting, Boston, MA, 2013.

Grigoryan B, Balaoing LR, Tseng H, Raphael RM, Killian TC, Souza GR, Grande-Allen KJ. A 3D co-culture model of the aortic valve using magnetic levitation. Biomedical Engineering Society Annual Meeting, Atlanta, GA, 2012. 

Tseng H, Raphael RM, Killian TC, Souza GR. Assembly of a lung co-culture model using magnetic levitation. Gulf Coast Consortia Lung Research Day. Houston, TX, 2012.

Ayoub S, Tseng H, Shah JV, Cuchiara ML, West JL, Grande-Allen KJ. Investigating electrospinning as a method to induce anisotropic mechanical behavior in hydrogels used for heart valve tissue engineering. Biomaterials Day, Houston, TX, 2012.

Tseng H, Balaoing LR, Grigoryan B, Saltarrelli JG, Nandi I, Desai PK, Raphael RM, Killian TC, Souza GR, Grande-Allen KJ. The development of a 3D co-culture model of the aortic valve for the study of the effect of shear stress on biglycan production and structure. 9th World Biomaterials Congress, Chengdu, China, 2012.

Balaoing LR, Post AD, Lin AY, Tseng H, Nolasco L, Turner NA, Moake JL, Grande-Allen KJ. Biomechanical stimuli effects on valve endothelial cell anti-thrombotic mechanisms. 5th Biennial Conference on Heart Valve Biology and Tissue Engineering, Mykonos, Greece, 2012.

Tseng H, Kim EJ, Connell PS, Ayoub S, Shah JV, Grande-Allen KJ. Quantification of the role of glycosaminoglycans on the tensile and viscoelastic properties of aortic valve leaflets. 5th Biennial Conference on Heart Valve Biology and Tissue Engineering, Mykonos, Greece, 2012.

Tseng H, Balaoing LR, Grigoryan B, Saltarrelli JG, Nandi I, Desai PK, Raphael RM, Killian TC, Souza GR, Grande-Allen KJ. The development of a 3D co-culture model of the aortic valve for the study of the effect of shear stress on biglycan production and structure. Southern Biomedical Engineering Conference, Houston, TX, 2012.

Tseng H, Ayoub S, Kim EJ, Shah JV, Cuchiara, ML, West JL, Grande-Allen KJ. Anisotropic hydrogel-fiber composites for heart valve tissue engineering. Tissue Engineering and Regenerative Medicine International Society–North America Annual Conference and Exposition, Houston, TX, 2011.

Tseng H, Raphael RM, Killian TC, Souza GR. Assembly of a lung co-culture model using magnetic levitation. Biomedical Engineering Society Annual Meeting, Hartford, CT, 2011.

Tseng H, Souza GR, Grande-Allen KJ. A three-dimensional two cell co-culture model of the aortic valve using magnetic levitation. Biomaterials Day at Texas A&M University, College Station, TX, 2011.

Tseng H, Grande-Allen KJ. The 3D elastin network of the aortic valve leaflet and its role in tissue mechanics. Biomedical Engineering Society Annual Meeting, Pittsburgh, PA, 2009.

 

Awards

American Heart Association Predoctoral Fellow, South Central Affiliate - Jan. 2011 - Jan 2013

IBB Mary Frances Dunnam Graduate Fellow - May 2011

Outstanding Teaching Assistant in Bioengineering - May 2011

NIH Biotechnology Training Fellow - Jan. 2008 - Sep. 2010

Vredenburg Scholar - Jun. 2006

Pi Tau Sigma - May 2006

 

Activities

 

Valhalla - Volunteer Bartender - Jan 2008 - Aug 2015

Rice GSA Dragon Boat - Captain - May 2008 - May 2012

Rice Bioengineering GSA - Social co-chair - May 2008 - May 2010

Pi Tau Sigma, Tau Alpha Chapter - President - May 2006 - May 2007

Languages: Fluent in Mandarin , Conversational in Cantonese

Professional Societies: American Society of Mechanical Engineers, Biomedical Engineering Society, Society for Biomaterials, Tissue Engineering and Regenerative Medicine International Society, American Association of Pharmaceutical Scientists